Supplier: OMEGA BIO-TEK

Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

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For simple and rapid homogenisation of cell and tissue lysates.

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By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.

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RNA isolation, total RNA Midi kit, E.Z.N.A.®

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The E.Z.N.A.® Yeast RNA kit is designed for isolation of high quality total RNA from a wide variety of yeast species. Up to 5×10⁷ log-phase yeast cultures can be processed for each column. Using an enzyme/beads-beating procedure to efficiently break the cell, combined with an optimised cell lysis buffer, the E.Z.N.A.® Yeast RNA kit can purify high-quality of total cellular RNA from various yeast strains.

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Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

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The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

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Selected protease digestion releases microgram amounts of DNA from FFPE samples.

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Nucleic Acid Purification Kits and Reagents, MicroElute® DNA columns

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Accessories for MagBinder® Fit24 Nucleic Acid Purification System

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Binding buffer XP2, Omega Bio-Tek

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Mag-Bind® Viral DNA/RNA Xpress Kit CE IVD follows a magnetic bead-based approach for the rapid and reliable isolation of viral DNA and RNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM), from saliva and other sample sources.

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The Mag-Bind® Blood DNA HV Kit CE IVD is designed for rapid and reliable isolation of high-quality genomic DNA from up to 4 ml whole blood samples.

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Automation-ready, prefilled 96-well plates for quick and convenient DNA extraction from blood, saliva, cultured cells, and swabs for magnetic processors.

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Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

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Taq DNA polymerase is a thermostable enzyme that can withstand prolonged incubation at temperatures up to 95°C without significant loss of activity.

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The E.Z.N.A.® Water DNA kit is formulated to isolate high purity cellular DNA from water samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including tap water, lake, river and sewage samples. Isolated DNA can be used for most downstream applications including PCR, Southern blotting, SNP analysis.

Supplier: OMEGA BIO-TEK

The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

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The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

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DNA gel loading buffer

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E.Z.N.A.® MicroElute® Genomic DNA kit provides a rapid and easy method for the isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results. Using the reversible binding properties of a silica-based material, the speed of mini-column spin technology, and specifically formulated buffers, the system allows the purification of genomic DNA up to 60 kb. Samples are first lysed under denaturing conditions and then applied to the HiBind MicroElute® Column to bind DNA, while cellular debris, haemoglobin, and other proteins are effectively washed away. High quality DNA can be eluted with 10 to 15 µl sterile deionised water or elution buffer from the MicroElute® column.

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Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

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Mag-Bind® Viral DNA/RNA kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from whole blood, serum, plasma, saliva and other body fluids. The Mag-Bind® magnetic beads technology enables purification of high quality nucleic acids that is free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down, allowing for the purification from various amounts of starting materials. The purified nucleic acid is ready for direct use in downstream applications, such as amplification or other enzymatic reactions.

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These dNTP solutions are suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabeled dNTPs.

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The 1 kbp DNA marker is ideal for determining the size of double-stranded DNA within the range 1 kbp to 10 kbp. The ladder consists of eight fragments and is pre-mixed with loading dye and ready to use.

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Modified from the E.Z.N.A.® Plant RNA kit, the E.Z.N.A.® Fungal RNA kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind® RNA spin columns, this method permits purification of high quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, northern blotting, Poly(A)+ mRNA purification, and differential display.

Supplier: OMEGA BIO-TEK

The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.

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96-well PP collection plates with 2,0 ml well volume for lysis, sample cultivation, and flow-through collection. This square well plate allows for larger volume than the equivalent round well plate in a standard microplate format.

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Mag-Bind® RXNPure Plus Kit allows rapid and reliable isolation DNA from PCR and enzymatic reactions with high recovery rates. The system combines proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads that selectively bind PCR amplicons 100 bp and larger, and eliminate excess nucleotides, primers and small, non targeted amplification products, such as primer dimers.