74293 Results for: "MP Biomedicals"

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Lymphocyte Separation Medium (LSM™) is formulated for isolation of mononuclear cells from defibrinated or heparinised whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets. It is a sterile-filtered solution of a sucrose polymer and diatrizoate salts.

Supplier: MP Biomedicals

Ratchet nut, blue, v3.0, For: FastPrep-24 homogenisers

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Readily soluble in water.

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Ursolic Acid is a naturally occurring triterpenoid, has been noted to demonstrate anti-angiogenic and anti-proliferative properties.

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Polyvinylpyrrolidone is a water-soluble polymer made from the monomer N-vinylpyrrolidone. Typically used as thickeners, dispersing agents, detoxicant, complexing agent, lubricants and binders.

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(-)-trans-Caryophyllene is a natural bicyclic sesquiterpene that is a constituent of many essential oils.

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β-Alanine is an N-blocked form of alanine. A nonselective endogenous glycine receptor agonist; a taurine analogue; an electrolyte.

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Tannic Acid is used for enzyme immobilization and protein adsorption

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Agarose High Resolution is an intermediate melting agarose with approximately twice the resolution capability of routine agarose. It is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. It is ideal for the resolution of small DNA fragments in the range 20 bp to 2000 bp.
Agarose high resolution is an intermediate melting and gelling point agarose which exhibits superior sieving characteristics. It is recommended for analytical gels of DNA fragments under 1000 bp and is ideal for small DNA fragments down to 20 bp can be separated on 6 % gels. It resolves fragments differing in size by only 2 bp. Its use avoids the need for PAGE Southern and Northern transfers can be achieved efficiently from them at concentrations up to 2%. DNA fragments can be purified with high efficiency from this agarose gels up to 4% by using either a purification kit or an electroelution system.

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Polymyxin B sulphate is a mixture of Polymyxin B1 and Polymyxin B2 sulphate, obtained from Bacillus polymyxia strains. Polymyxin B inhibits gram-negative organisms in vitro wherein it binds to and interferes with the permeability of the bacterial cytoplasmic membrane and is also reported to inhibit Ca²⁺ dependent protein kinase C (PKC). Effective concentration is typically 50 mg/l.

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ε-Amino-n-caproic acid is a plasmin inhibitor. It inhibits carboxypeptidase B. It promotes rapid dissociation of plasmin by inhibiting the activation of plasminogen. As an inhibitor of plasmin it is has been utilised in the clotting buffer for fibrinogen assays.

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A chromogenic substrate for alkaline phosphatase.

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IBMX has been shown to be a potent, non-specific inhibitor of adenosine 3',5'-cyclic monophosphate phosphodiesterase (cAMP PDE)4, significantly more effective than theophylline. Also inhibits cGMP phosphodiesterases. IBMX inhibits cyclic nucleotide PDE with subsequent inhibition of cyclic nucleotide hydrolysis, resulting in accumulation of cyclic AMP and guanosine 3',5'-cyclic monophosphate.

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Silicon dioxide, Ecochrom™ 32-63 µm

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Protein kinase C inhibitor (IC50 = 50-200 µM depending on assay conditions in MCF-7 cells or IC50 = 100 µM in rat brain). The PKC inhibition is also dependent on the phosopholipid concentration. Also inhibits both calmodulin-dependent and calmodulin-independent Ca2+-, Mg2+-ATPase. Tamoxifen is a selective estrogen response modifier (SERM), anti-angiogenetic factor. It is a prodrug that is metabolized to active metabolites 4-hydroxytamoxifen (4-OHT) and endoxifen by cytochrome P450 isoforms CYP2D6 and CYP3A4. In breast cancer, the gene repressor activity of tamoxifen against ERBB2 is dependent upon PAX2. Blocks estradiol-stimulated VEGF production in breast tumor cells.
Tamoxifen has been used to facilitate the recombination of ect2flox allele in mouse organs. It has also been used to study its effect on lipopolysaccharide (LPS)-induced microglial activation
Tamoxifen is a Protein kinase C inhibitor. It induces apoptosis in human malignant glioma cell lines. Tamoxifen and its metabolite 4-hydroxytamoxifen are selective estrogen response modifiers (SERMs) that act as estrogen antagonists in mammary gland. Blocks estradiol-stimulated VEGF production in breast tumor cells.

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Each impact-resistant tube contains 1,6 mm aluminum oxide particles and 1,6 mm silicon carbide particles.

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Eosin Y disodium salt is used in combination with Azure II to show morphological details of marrow cells; buffered Azure A/Eosin Y method for demostrating cell granules, microorganisms and nuclei; for showing insect musculature; for demonstrating cells infected with mosquito-iridescent virus; use in combination with Alum Hematoxylin/Azure II to show mast cells, early necrobiotic changes in cells and protozoans, rickettsias and bacteria in tissues; to demonstrate volutin granules in any microorganism; Bond's modification of Mann's Eosin-Methyl Blue for Negri bodies in smears; Harris stain for Negri bodies in smears; Mann's method for staining Negri bodies in sections; Gridley's stain for Endamoeba in tissue sections; a modified methenamine-silver nitrate method for the demonstration of Endamoeba in sections. It is used as a counterstain for hematoxylin.

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Hexadecane has been used as a phase change material (PCM) in the preparation of hexadecane/xGnP (exfoliated graphite nanoplatelet), a composite PCM loaded with xGnP.

Supplier: MP Biomedicals

SPINeasy DNA Kit for Water is a high-performance water genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from water in less than 30 min. Water samples are processed using our uniquely formulated Lysis Buffer W1 and Lysing Matrix E to effectively lyse various types of cells. Column W1 provided in the kit has high binding capacity and selectivity for gDNA. The combination of components in the kit extracts gDNA of high yield and purity that is ready for downstream analyses such as PCR, restriction digestion and sequencing.

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Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. It exists as a dimer below pH 5,0 and and at pH >7 it exists as a tetramer. It is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding.

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The methylated xanthine compounds caffeine, theobromine, and theophylline and their derivatives are used in medicine for their bronchodilator effects.

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Rotenone is a naturally occurring botanical compound obtained from the roots of Derris sp.& Cube. It is an inhibitor of mitochondrial electron transport, specifically inhibiting NAD-linked substrate oxidation by mitochondria at the oxygen side of NADH dehydrogenase.
Rotenone is utilized as an insecticide. It can be used as neurotoxic agent that can produce a Parkinson-like condition as an animal model for study of etiology and interventions.
Inhibitor of mitochondrial electron transport at NADH: ubiquinone oxidoreductase. It is readily absorbed through the exoskeletons of arthropods, but poorly absorbed cutaneously or from the gastrointestinal tract of mammals. Rotenone is used to induce a Parkinson-like syndrome as an experimental model in rats.

Supplier: MP Biomedicals

Applications
Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA. It is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA.
Product Description
The crystal structure of Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds,it is a strong chaotropic agent.Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a freeflowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical

Grade: Ultra Pure
Purity: >99%
Keywords: Guanidium chloride, chaotropic agent
Key Applications: Chaeotropic agent
Product Type: Biochemicals
Biochemical Category: Chaotropic Agents
Density: 1.345 g/cm³ at 20 °C (Lit.)
Melting Point: 180-190 °C
UV/Visible Absorbance: OD260nm (6.0 M aq soln) <0.03
Presentation: White Crystalline Powder
pH: 4-6 (6.0 M aq soln)
Solubility: Soluble in water,Clear, Colorless Solution (6 M - clear, colorless solution).
Storage & Handling: Room Temperature, desiccate

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Linolenic acid belongs to the class of omega-3 fatty acids.

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Conduritol B Epoxide (CBE) is a potent, irreversible inhibitor of various plant β-glucosidases and mammalian glucocerebrosidase, specific covalent inhibitor of β-glucosidase which results in the accumulation of glucocerebroside.

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Soluble in water.

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Solubility (5% aq soln) clear, colorless solution.

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3-Methylxanthine, a metabolite of theophylline, was quantitated in rat plasma by a sensitive LC-MS/MS method.

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Collagen is Type I soluble, salt free component of skin, bone, tendon, and other fibrous connective tissues. Type I collagen differs from other collagens by their low lysine hydroxylation and low carbohydrate composition. It breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

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Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. A compromise between the easier to use lower molecular weight products and the extremely viscous higher molecular weights would be the products in the range of 70,000-150,000.
It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.
Other uses for poly-lysine have been reported as well:
• Conjugation to methotrexate for increased drug transport.
• Microencapsulation of islets.
• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.
• Immobilation of living cells in biocompatible semipermeable microcapsules.
• In the preparation of polycationic beads.
• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Poly-D-lysine hydrobromide is a synthetic amino acid that enhances cell adhesion to solid substrates. It has additionally been shown to eliminate prion proteins from infected cells.