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Wheat germ agglutinin (WGA) conjugates

Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

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One-Step Blue® protein gel stain

One-Step Blue® protein gel stain

Supplier: Biotium

One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.

   Sustainable Options Available
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Nucleic acid stains, PAGE GelRed™

Nucleic acid stains, PAGE GelRed™

Supplier: Biotium

Stains specifically designed for DNA in polyacrylamide gels.

   Sustainable Options Available
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GelRed® & GelGreen® Nucleic acid gel stains

GelRed® & GelGreen® Nucleic acid gel stains

Supplier: Biotium

GelRed and GelGreen are highly sensitive DNA/RNA gel stains made safe by preventing penetration into cells.

   Sustainable Options Available
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EvaGreen® Plus dye, 20X in Water

Supplier: Biotium

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

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Propidium iodide 1 mg/ml in water

Propidium iodide 1 mg/ml in water

Supplier: Biotium

Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

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SYBR® Green I 10000x in DMSO nucleic acid gel stain

Supplier: Biotium

Thiazole green, which is structurally identical to SYBR® green I nucleic acid gel stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

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PMAxx™ dye 20 mM in water

PMAxx™ dye 20 mM in water

Supplier: Biotium

PMAxx™ dye is a DNA modifier used for viability PCR (v-PCR). V-PCR is a powerful PCR-based, culture-independent, method for rapid and sensitive quantification of microbial viability.

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Decontamination bags, activated charcoal

Decontamination bags, activated charcoal

Supplier: Biotium

Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.

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PMA (Propidium monoazide) DNA/RNA binding dye

PMA (Propidium monoazide) DNA/RNA binding dye

Supplier: Biotium

PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.

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Fluorometric polymerase activity assay kit, EvaEZ™

Supplier: Biotium

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

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qPCR master mixes, Forget-Me-Not™ EvaGreen®

qPCR master mixes, Forget-Me-Not™ EvaGreen®

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

   Sustainable Options Available
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Cell scrapers

Cell scrapers

Supplier: Biotium

PE, disposable, sterile. Mini cell scrapers for harvesting cells or cell lysates from microwell plates.

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Lipofuscin autofluorescence quencher, TrueBlack™

Supplier: Biotium

TrueBlack™ is a reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a cell and tissue types with age.

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