23842 Results for: "Assay Kits and Reagents"

Supplier: Abcam

FirePlex® miRNA Assay Core Reagent Kit V2 - Biofluid 1 * 1 KIT

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: Abcam

FirePlex+reg miRNA Assay Core Reagent Kit V2 - FFPE and Tissues 1 * 1 KIT

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

Supplier: Merck Millipore (Calbiochem‎)

Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.

Supplier: Thermo Fisher Scientific

The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Supplier: Abcam

FirePlex® miRNA Assay Core Reagent Kit V2 - purified RNA 1 * 96 Tests

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

Supplier: ENZO LIFE SCIENCES

A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

Supplier: G-Biosciences

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

Supplier: Thermo Fisher Scientific

The Pierce™ HA-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

Supplier: Thermo Fisher Scientific

The Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

Supplier: G-Biosciences

The femtoELISA™ kits utilise a non animal protein blocker, NAP-BLOCKER™ that minimises cross-reactivity with researcher's antigens and antibodies. For the detection of HRP, an improved, ultra sensitive, non volatile, stable colorimetric substrate based on tetramethyl benzidine (TMB) is supplied. Reagent does not require hydrogen peroxide that can have detrimental effects on many biological factors, which may ultimately affect the assays.

Supplier: G-Biosciences

Kit Protein Fractionation includes tissue extract & pestle, protein buffer, precipitant-Mes, salt solution (ammonium sulfate), prote in assay: CB reagent, protein assay: protein standard (2mg/ml BSA), centrifuge tubes (1,5ml). 1 * 1 KIT

Supplier: Thermo Fisher Scientific

The Compat-Able™ Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 min with just a few simple steps. The Compat-Able™ Preparation Set is available conveniently bundled with the Pierce BCA™ Protein Assay Kit.

Supplier: Biosensis

The LR³IGF-I Rapid™ ELISA Kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR³IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR³IGF-I capture antibody pre-coated onto an ELISA plate, standard LR³IGF-I protein, a biotinylated anti- LR³IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of LR³IGF-I present in samples and the supplied protein standard.

Supplier: Biosensis

The Biosensis ApoE/Aβ Complex ELISA kit is a sandwich ELISA that allows the preferential measurement of ApoE/Aβ complexes. This kit consists of a pre-coated mouse monoclonal anti-Aβ capture antibody, a highly validated ApoE/Aβ complex standard that is pre-formed, lyophilised and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the level of ApoE/Aβ complex present in samples and protein standards. Importantly, a well-characteriaed and unique ApoE/Aβ complex is included as a standard. This complex is pre-formed and lyophilised, requiring only reconstitution with assay diluent prior to use.

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: Merck Millipore (Novagen)

The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

Supplier: ENZO LIFE SCIENCES

BIOARRAY™ terminal labelling kit with Biotin-ddUTP has been developed for DNA probe array assays.

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

Supplier: CELL VIABILITY

The Cell Viability Reagent Kits are designed specifically for use with the Beckman Coulter Vi-CELL® Cell Viability Analyser. The Cell Viability Reagent Kits, as well as being cost-efficient, are proven to be of equivalent quality and performance when compared to Beckman Coulter Vi-CELL® Kits.

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.