SPINeasy® DNA Kit for Tissue (with Lysing Matrix) is a high-performance genomic DNA (gDNA) extraction kit which combines mechanical, chemical and enzymatic disruption of tissue samples in 30 minutes. The addition of proteinase K allows a more efficient and complete digestion of any tissue type. Homogenizing time is greatly reduced while substantially increasing the gDNA yield and purity. The kit is validated for tissue samples of fatty, tough, and fibrous samples, and suitable for downstream applications such as PCR and qPCR.

Hard-to-break samples including fatty and tough fibrous tissues (e.g., tendons or ligaments) pose significant challenges in the first step of DNA isolation. Conventional ways of sample rupture or enzyme digestion may not completely lyse those challenging samples.

Components: Lysing matrix M (50 ea), equilibration buffer (12 ml), buffer TD1 (15 ml), buffer TD2 (30 ml), buffer TD3 (18 ml), buffer TD4 (6 ml), buffer TD5 (15 ml), proteinase K (1,2 ml), RNase A (250 µl), column S (50 ea), collection tube (150 ea), elution tube (50 ea).