The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

• Capped with CleanCap® AG cap analog, no base modifications in the sequence

  Cap 1 structure with >95% capping efficiency for superior in vivo activity compared to Cap-0 mRNAs

  Manufactured with TriLink’s proprietary CleanScript® method to lower dsRNA for improved performance

  Ready to use in downstream mRNA applications and formulations

  Grade: RUO

This mRNA is capped using CleanCap® Reagent AG, TriLink's co-transcriptional capping technology, which results in the naturally occurring Cap-1 structure with >95% capping efficiency. It is manufactured using TriLink’s CleanScript® method, polyadenylated, and optimised for mammalian systems. It mimics a fully processed mature mRNA.

CleanCap Reagent AG produces a base-modified Cap-1 mRNA, which shows superior in vivo activity compared to Cap-0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).

CleanScript method is TriLink’s proprietary in vitro transcription process that is optimised to minimise dsRNA formation and increase mRNA activity in vivo.