- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:Glycosaminoglycan
- Description:Glycosaminoglycan ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Glycosaminoglycan ELISA Kit exhibits high specificity and excellent specificity for the detection of universal Glycosaminoglycan. No significant cross-reactivity or interference between Glycosaminoglycan and analogues was observed.
- Detection method:Colorimetric
- Time to Results:2 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:1,563 - 100 ng/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:0,938 ng/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Glycosaminoglycan ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Glycosaminoglycan in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Glycosaminoglycan ELISA Kit employs the competitive enzyme immunoassay technique for the quantitative measurement of Glycosaminoglycan in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Glycosaminoglycan antigen. During the incubation, Glycosaminoglycan present in the samples or standards competes with the fixed amount of immobilised Glycosaminoglycan for binding sites on the Biotinylated Anti-Glycosaminoglycan Antibody. The more Glycosaminoglycan present in a sample or standard, the less Biotinylated Anti-Glycosaminoglycan Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Glycosaminoglycan Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Glycosaminoglycan present in each sample or standard. The concentration of Glycosaminoglycan can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.