Mouse MCP2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse MCP2 in serum, plasma, and other biological fluids. This ELISA kit only requires 25 µl of sample volume(s).

• Small Sample Volume: Requires as little as 25 µl of sample!
• Assay Precision: Intra-Assay: CV <10%, Inter-Assay: CV <10%

Mouse MCP2 ELISA kit employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse MCP2 in serum, plasma, and other biological fluids. An antibody specific for MCP2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the MCP2 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-MCP2 Antibody, which binds the captured MCP2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of MCP2 captured in each well. The concentration of MCP2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.