Human Kininogen 1 ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Kininogen 1 in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay Precision: Intra-assay CV: 3,6%, Inter-assay CV: <10%

Human Kininogen 1 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Kininogen 1 in serum, plasma or other biological fluids. An antibody specific for Kininogen 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Kininogen 1 present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-Kininogen 1 Antibody, which also binds the Kininogen 1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Kininogen 1 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Kininogen 1 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Kininogen 1 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Kininogen 1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.