The Corning® BioCoat® Angiogenesis systems facilitate investigation of compound effects on endothelial cell invasion, migration, and tubulogenesis. The availability of these standardised assays has facilitated a better understanding of the molecular mechanism of angiogenesis and simplified the routine use of cell based assays for screening of anti- and pro-angiogenic compounds.

• Address key steps in the angiogenesis process using standardised cell-based assays
• Increase throughput with screening-compatible formats and data acquisition options
• Obtain human umbilical vein endothelial cells pre-qualified for use in the endothelial cell migration assay

Corning® BioCoat® Angiogenesis system: Endothelial Cell Invasion
For evaluation of endothelial cell invasion using real time fluorescence detection in a simplified and reproducible manner.

System includes 24-multiwell insert plate(s) with 3 μm pore size Corning® FluoroBlok™ fluorescence-blocking PET membrane pre-coated with Corning® Matrigel® Matrix, packaged ready-to-use with Falcon® companion plate.

Corning® BioCoat® Angiogenesis system: Endothelial Cell Migration
A quantitative and reproducible in vitro model system for examining the effects of prospective compounds on endothelial cell migration.

System includes 24- or 96-multiwell insert plate(s) with 3,0 μm pore size Corning® FluoroBlok™ fluorescence-blocking PET membrane pre-coated with human fibronectin, packaged ready-to-use with Falcon® companion plate.

Corning® BioCoat® Angiogenesis system: Endothelial Cell Tube Formation
An optimised system for screening compounds that modulate endothelial cell tubulogenesis, which saves time and improves reproducibility.

System includes 96-well black plate with clear bottom uniformly coated with Corning® Matrigel® Matrix, packaged ready-to-use with Falcon® companion plate.

Corning® Human Umbilical Vein Endothelial Cells
Corning® Human Umbilical Vein Endothelial Cells (HUVEC-2) are derived from single donors and cryopreserved at passage number 2. HUVEC-2 cells have been pre-qualified to assure a robust migratory response to angiogenic factors such as VEGF and FBS. Single donor primary HUVEC-2 cells are suitable for use in combination with Corning® BioCoat™ Angiogenesis systems to provide relevant models for angiogenesis (e.g. cardiovascular, vascular, and wound healing) and cancer research. Corning® Human Umbilical Vein Endothelial Cells have been qualified for use in Corning® BioCoat™ Angiogenesis Endothelial Cell Migration assays and may be used in Corning® BioCoat™ Endothelial Cell Invasion and Tube Formation assays.

A quantitative and reproducible in vitro model system for examining the effects of prospective compounds on endothelial cell migration.