Specifications
- Detector:Two matched pairs of photomultiplier tubes (PMTs), optimised for different detection modes
CCD spectrometer - Light source:High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET - Wavelength selection:Full spectra or 8 distinct wavelengths in <1 sec/well
- Description:PHERAstar® FSX HTS plate reader
- Electrical:100 or 115 or 230 V, 50/60 Hz, auto ranging
- Read time (fly mode):1 Flash: 14 s (384), 27 s (1536)
10 Flashes: 38 s (384), 1 min 52 s (1536)
50 Flashes: 1 min 29 s (384), 5 min 18 s (1536) - Dimensions:450W×510D×470H mm (480W×680D×470H mm with AAS system)
- Measuring modes:Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning - Shaking:Linear, orbital, and double-orbital with user-definable time and speed
- Software:Multi-user reader control and MARS Data analysis software included
FDA 21 CFR Part 11 compliant - Weight:49 kg (62 kg with AAS)
- Incubation range:+5 °C above ambient up to 45 °C
- Pk:1
Specifications
About this item
The PHERAstar® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. The unique Optic Module system, Simultaneous Dual Emission, Enhanced Dynamic Range technology, UV/vis spectrometer, AAS System, and dedicated AlphaScreen® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.
- Most sensitive reader in fluorescence intensity and polarisation
- The only plate reader with 5 assay-dedicated detectors
- Simultaneous Dual Emission, including for Alpha Technology
- Enhanced Dynamic Range and automatic focus
- 9 decades luminescence dynamic range
- Dedicated AlphaScreen®/AlphaLISA®/AlphaPlex™ laser
- New generation TRF-laser and advanced assay stability system
- Up to three integrated barcode readers
Top and bottom focal height adjustment. High-precision injectors with simultaneous reagent injection and detection. High-speed absorbance measurements.
Detection modes: Fluorescence intensity - incl. FRET, Fluorescence polarisation, AlphaScreen®/AlphaLISA®/AlphaPlex™, Luminescence (flash and glow) - incl. BRET, Time-resolved fluorescence, TR-FRET, UV/Vis absorbance spectra.
Microplate formats: Up to 3456-well plates, user-definable, LVis Plate with 16 low volume microspots (2 μl).
Microplate carrier: Robot compatible
Spectral range:
Filters
230 to 750 nm or 230 to 900 nm for FI, FP
230 to 750 nm for LUM
230 to 900 nm for TRF
Spectrometer
220 to 1000 nm for ABS; wavelength precision: ≤0,5 nm