EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kits

Supplier: STEMCELL Technologies

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Supplier: STEMCELL Technologies

About this item

Immunomagnetic selection of human CD4+CD127lowCD25+ regulatory T cells using particle release technology

Highly purified human CD4+CD127lowCD25+ Tregs isolated in less than 1 hour
No-wash removal of EasySep™ Releasable RapidSpheres™
Optional isolation of CD4+CD25- responder T cells from the same sample

This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Caution: Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier’s restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').