Immunomagnetic positive selection of CD11b+ cells from human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples.

• Fast and easy-to-use
• Up to 99% purity
• No columns required

Efficiently isolate or deplete human CD11b+ cells from fresh or previously frozen peripheral blood mononuclear cells in as little as 16 minutes. Cells isolated with the EasySep™ Human CD11b Positive Selection and Depletion Kit can be used immediately in downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

Use this kit to label CD11b+ cells with antibodies and magnetic particles. The cells are then subsequently separated—without the use of columns—using an EasySep™ magnet. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction can be retained for subsequent applications.

This kit targets human CD11b, a subunit of the integrin macrophage-1 antigen (Mac-1) receptor commonly used as a myeloid cell marker. CD11b is expressed on immune cells such as monocytes, macrophages, neutrophils, and NK cell subsets, and is known to play a role in regulating cell adhesion, migration, and phagocytosis