Human CCL4/MIP-1 beta ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CCL4/MIP-1 beta in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human CCL4/MIP-1 beta ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CCL4/MIP-1 beta in serum, plasma or other biological fluids. An antibody specific for CCL4/MIP-1 beta has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CCL4/MIP-1 beta present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-CCL4/MIP-1 beta antibody, which also binds the CCL4/MIP-1 beta present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-CCL4/MIP-1 beta antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-CCL4/MIP-1 beta antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of CCL4/MIP-1 beta captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of CCL4/MIP-1 beta in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.