Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:GAD67
- Description:Mouse GAD67 ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Mouse GAD67 ELISA kit exhibits high specificity and excellent specificity for the detection of mouse GAD67. No significant cross-reactivity or interference between GAD67 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,391 - 25 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,234 ng/ml
- Regulatory status:RUO
Specifications
About this item
Mouse GAD67 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GAD67 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0,391 to 25 ng/ml
- Sensitivity: 0,234 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse GAD67 ELISA kit (A78123) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse GAD67 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for GAD67 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GAD67 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GAD67 Antibody, which binds the captured GAD67 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GAD67 captured in each well. The concentration of GAD67 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.