Gel filtration column for purification and desalting of proteins larger than 5 kDa or nucleic acids longer than 10 bp/nt.

CentriPure gel filtration columns are designed for rapid and efficient removal of small molecules such as dyes, salts, biotin, ammonia, haptens, etc. from larger nucleic acids, proteins or nanoparticles, which are simultaneously purified and desalted in a single step.

The solid phase in CentriPure 2, 5, 10 - Z25M is Zetadex-25, a size-exclusion gel that is suitable for approximately 10 base pairs for nucleotides and 5 kDa for globular proteins. It may also be used with spheroidal nanoparticles greater than 2 nm in diameter. Entities larger than the pores are excluded from the gel, remain in the void volume, pass rapidly through the column, and are eluted free of low molecular weight contaminants. The removal efficiency of small molecules typically exceeds 99,99%, with a dilution of around 1,5 to 2-fold, and a nucleotide or protein recovery of greater than 85%.

Zetadex-25 medium is a cross-linked beaded dextran solid phase with an average particle size of 50 to 150 µm. The particle size range enables purification in gravity-based formats. CentriPure columns can be pre-washed with pure water for desalting or pre-equilibrated with a buffer of choice for a customised buffer exchange. The gravity column provides a significantly faster and far more efficient alternative to dialysis or TFF. Samples are processed in less than 15 minutes.