Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.

• Efficient and easy to use - kit reagents and gradient centrifugation separate organelles from contaminating structures
• Compatible - prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localisation studies
• Complete - kits contain sufficient material for 25 applications

Subcellular fractionation simplifies otherwise complex protein mixtures that result from whole cell lysis, thereby facilitating proteomic analysis of specific organelle targets and pathways. Each kit uses density gradient centrifugation to separate organelles from contaminating cellular structures. The isolated organelles may be used for a number of downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localisation studies.