Animal component-free medium for human mesenchymal stem cells.

Reduce variability in human mesenchymal stromal cell (MSC; also known as mesenchymal stem cell) cultures and improve experimental reproducibility by using this animal component-free (ACF) and extracellular vesicle (EV)-free medium. MesenCult™-ACF Plus Medium is optimised to derive human MSCs from multiple sources, such as bone marrow or adipose tissue, without serum. Compared to serum-containing or EV-depleted serum-containing media, MSCs cultured with this kit expand more efficiently (rate and cumulative cell total) and without compromising function.

The cultured cells exhibit characteristic MSC surface marker expression and retain robust expansion rate and trilineage differentiation capacities. MesenCult™-ACF Plus Medium Kit is specifically designed for deriving, expanding, and cryopreserving MSCs, as well as differentiating human pluripotent stem cells into mesenchymal progenitors - that is optimised for efficient and consistent MSC cultures. For animal component-free and optimised cryopreservation, MesenCult™-ACF Freezing Medium is recommended for human MSCs previously cultured in MesenCult™ media, including MesenCult™-ACF Plus.

NOTE: Complete MesenCult™-ACF Plus Medium must be supplemented with L-Glutamine. It is also intended to be used in conjunction with Animal Component-Free Cell Attachment Substrate, which is available as part of the MesenCult™-ACF Plus Culture Kit. CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.