Kit including BrainPhys™ Neuronal Medium and SM1 Neuronal Supplement for culture of primary and ES/iPS cell-derived neurons.

Culture primary or human pluripotent stem cell (hPSC)-derived neurons long term in a medium optimised to promote, rather than inhibit neuronal activity and maturity. For your convenience, BrainPhys™ Neuronal Medium and SM1 Kit includes both serum-free BrainPhys™ Neuronal Medium (basal medium) and NeuroCult™ SM1 Neuronal Supplement, a Brewer's B27-based supplement that ensures cell health and encourages neurite outgrowth and branching in short- and long-term serum-free cultures.

Based on the formulation, BrainPhys™ Neuronal Medium mimics the extracellular environment of the central nervous system (CNS) to yield a higher proportion of synaptically active neurons. Use BrainPhys™ Neuronal Medium and SM1 Kit to culture primary CNS-derived neurons or embryonic stem (ES) and induced pluripotent stem (iPS) cell-derived neurons. To avoid shocking cells with media changes, also use this culture system when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.