Serum-free and sex steroid-modular kit for the expansion and differentiation of human fallopian tube organoids.

Generate mature fallopian tube (FT) organoids that exhibit a representative combination of ciliated and secretory cells as they appear in vivo. GyneCult™ fallopian tube organoid medium (FTOM) outperforms do-it-yourself (DIY) formulations by supporting the superior growth of functional FT organoid cultures with physiological proportions of relevant cell types. Organoids generated with GyneCult™ FTOM provide a robust and versatile system for female reproductive biology research. This optimised workflow cuts down on time and labor by seeding directly into 3D culture, enabling the growth of passageable FT organoids by day 10. GyneCult™ FTOM is a serum-free and sex steroid-modular kit, by omitting the addition of the 5000X supplement, adjust hormone levels as needed while delivering consistent results for specific applications. Applications of FT organoid cultures include the study of fertility, hormone dynamics, menstrual and post-ovulation cycles, and ovarian cancer. With GyneCult™ FTOM, a subset of high-grade serous ovarian cancer (HGSOC) organoids can be cultured for use in ovarian cancer research, such as evaluating the tumor initiation capacity of FT epithelial cells or screening of chemotherapy drugs for the treatment of ovarian cancer.