Transforming growth factor alpha is a protein that in humans is encoded by the TGFA gene. As a member of the epidermal growth factor (EGF) family, TGFa is a mitogenic polypeptide. The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling. TGFa is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. [Wiki].

Immunofluorescence: 1 - 2 µg/ml Immunohistochemistry (FFPE): 2-4 µg/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the TGF alpha antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10 mM citrate buffer for 10 - 20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. The epitope is known to be in the C-termial region of the protein.

Type: Primary
Antigen: TGFA
Clonality: Monoclonal
Clone: TFGFa-1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human