- Antibody type:Primary
- Antigen name:anoctamin 1, calcium activated chloride channel
- Antigen symbol:ANO1
- Clonality:Monoclonal
- Clone:DG1/1486
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b kappa
- Reactivity:Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:Q5XXA6
- Antigen synonyms:ANO1
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94,4% vs. 94,7%.
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml IF: 0,5 - 1 µg/ml IHC (FFPE): 0,5 - 1 µg/ml for 30 minutes at RT The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.
Type: Primary
Antigen: ANO1
Clonality: Monoclonal
Clone: DG1/1486
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human