Specifications
- Antibody type:Primary
- Antigen name:Friend leukemia virus integration 1
- Antigen symbol:FLI1
- Clonality:Monoclonal
- Clone:FLG/1562
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Reactivity:Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P20930
- Antigen synonyms:FLI1
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Amino acids 998-1104 of human FLG were used as the immunogen for this Filaggrin antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesised as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Flow Cytometry: 0,5 - 1 µg/10^6 cells in 0,1 ml IF: 1 - 2 µg/ml IHC (FFPE): 0,5 - 1 µg/ml for 30 min at RT Optimal dilution of the Filaggrin antibody should be determined by the researcher.
Type: Primary
Antigen: FLI1
Clonality: Monoclonal
Clone: FLG/1562
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human