- Antibody type:Primary
- Antigen name:immunoglobulin lambda locus
- Antigen symbol:IGL
- Clonality:Monoclonal
- Clone:ICO-106
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG1 kappa
- Reactivity:Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P01701; P01842
- Antigen synonyms:IGL
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Purified human Ig was used as the immunogen for this Lambda light chain antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
This antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Flow Cytometry: 0,5-1 µg/million cells IHC (FFPE): 0,5 - 1 µg/ml for 30 minutes at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6,0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Type: Primary
Antigen: IGL
Clonality: Monoclonal
Clone: ICO-106
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human