Stomach Lysate (7 Day Old)
PRSI1415-7
New Product
:
- Cell Format:Cryopreserved
- Pk:0,1 mg
- Description:Normal; Source: Mouse; Tissue: Stomach
- Environmentally Preferable:
- Storage conditions:Store at 2...8 °C for continuous use. For extended storage, freeze working aliquots at −70 °C.
- Storage buffer:Stomach lysate (7 Day Old) is supplied in SDS sample buffer containing 5% β-mercaptoethanol
- Concentration:2 mg/ml
- Tested applications:WB
Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.