Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes
EDVO212
: EDVOTEK
- Description:Cleavage of Lambda DNA with Eco RI Endonuclease: Intro to Restriction Enzymes
- Environmentally Preferable:
- Pk:1 KIT
The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.
- For 5 lab groups
- Complete in 90 minutes
Also required: Electrophoresis tank, power supply, 5 to 50 µl and 40 µl fixed volume micropipettes, water bath, and white light box.
: Kit includes instructions, Lambda DNA, Dryzymes™, reconstitution buffer, restriction enzyme reaction buffer, enzyme grade water, standard DNA fragments, various solutions and buffers, agarose powder, and stains.