A competitive ELISA kit for the quantitative measurement of 4-hydroxynonenal in biological fluids.

• Easy, convenient and time-saving method
• Detection Range: 31,25 to 2000 pg/ml
• Sensitivity: <18,75 pg/ml
• Assay precision: Intra-assay: CV <8%, inter-assay: CV <10%
• No significant cross-reactivity or interference was observed

4-hydroxynonenal (4-HNE) is a lipid peroxidation product derived from oxidised ω-6 polyunsaturated fatty acids such as arachidonic acid. 4-HNE is widely used as a marker of lipid peroxidation.

It is also in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction. It exhibits various biological activities such as cytotoxicity, growth inhibiting activity, genotoxicity, and chemotactic activity.

It also inhibits Sirtuin 3 (SIRT3) via thiol-specific modification.The 4-Hydroxynonenal ELISA kit is a competitive ELISA assay for the quantitative measurement of 4-HNE in serum, plasma and cell culture supernatants. The density of colour is proportional to the amount of 4-HNE captured from the samples.

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