About this item
Isolate plasmid DNA from up to 5 ml culture in 9 minutes utilizing an innovative lysate clearance column.
- Rapid - 9 Minute Processing time from a bacterial pellet
- Unique - Lysate Clearance and DNA Binding in 1 step
- Convenient - Lysate Clearance Column eliminates cellular debris pelleting and transfer step
- Versatile - Process up to 5 ml bacterial cultures with different plasmid types and culture medias
The E.Z.N.A.® fast filter plasmid DNA mini kit is designed for rapid purification of high-quality plasmid DNA starting with bacterial pellet from up to 5 ml of culture following the alkaline-lysis method in just 9 minutes. The kit features an innovative and first of its kind fast filter mini column nested within a regular HiBind® silica mini spin column to combine lysate clearance and DNA binding into one simple centrifugation step. Following wash and elute steps, purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Plasmid
- Sample type:Bacteria
- Target molecule:DNA
- Sample size:5 ml high-copy plasmid/10 ml low-copy plasmid
- Storage temperature:Dry at room temperature (20...25 °C)