Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  Fc Tag
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method
• Biological activity:
  Measured by its binding ability in a functional ELISA. Immobilised Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) at 5 μg/ml (100 μl/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0,4 - 6 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) at 10 μg/ml (100 μl/well) on Diamond Protein A Protein, His Tag precoated (0,5 μg/well) plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0,078 - 1,25 μg/ml (Routinely tested). Immobilised Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4,49 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0,78 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Anti-Siglec-15 MAb (Mouse IgG) on AMC Biosensor, can bind Human Siglec-15, Fc Tag (Cat. No.SG5-H5253) with an affinity constant of 59,7 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0,46 - 0,54 μg/ml (Routinely tested).
• Gene ID:
  Q6ZMC9-1
• Protein synonyms:
  Siglec-15|SIGLEC-15|Siglec15|CD33L3|CD33 antigen-like 3|sialic acid-binding Ig-like lectin 15
• Protein/peptide name:
  Siglec-15
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  53,0 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Shipping temperature:
  RT