Human Recombinant G-CSF (Animal free) (from E. coli)

Supplier: Shenandoah Biotechnology

Certificates

Supplier: Shenandoah Biotechnology

About this item

Persephin is a neurotrophic factor of the glial cell line-derived neurotrophic factor (GDNF) family. Persephin promotes survival and growth of dopaminergic and motor neurons, but not peripheral neurons. Persephin is a ligand for the RET receptor tyrosine kinase.

High quality
Low endotoxin
Affordable

G-CSF synthesis occurs in monocyte, macrophage, epithelial, endothelial, and fibroblast cells after activation by bacterial endotoxins, tumor necrosis factor alpha (TNF-α).

The functional activity of G-CSF is mediated through the granulocyte colony-stimulating factor receptor (G-CSF-R) to activate JAK/STAT and MAPK signal transduction pathways. G-CSF also promotes neurogenesis and inhibits neuronal apoptosis. Human and mouse G-CSF proteins are cross-reactive.

Delivery: A certificate of analysis (COA) is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions.

Caution: For research use only.

Specifications

Conjugation:
Unconjugated
Protein function:
Cytokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
−20 °C
Endotoxin content:
Endotoxin LAL (EU/µg) ≤0.1
Biological activity:
Bioactivity assay: NFS-60 cell proliferation, ED50 ≤0,060 ng/ml, bioactivity ≥1,7×10⁷ units/mg
Gene ID:
P09919
Reconstitution instructions:
Sterile water at 0,1 mg/ml
Endotoxin-free:
N
Carrier-free:
Y
Protease-free:
N
Animal-free:
Y
Protein synonyms:
GM-CSFβ|pluripoietin|MGI-1G|CSF-3
UniProtKB:
P09919
Protein/peptide name:
G-CSF
Purity:
≥95%
Molecular weight:
18,8 kDa
Sequence:
MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP
Endotoxin level:
Low
Formulation:
20 mM acetic acid, 50 mM sodium chloride
Nuclease-free:
N
Grade:
RUO
Shipping temperature:
RT
Tested applications:
Bioassay