Mouse Recombinant TNF-A (from E. coli)

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

High quality
Low endotoxin
Affordable

: For research use only.

Conjugation:
Unconjugated
Protein function:
Cytokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Mouse
Storage conditions:
–20 °C
Endotoxin content:
Endotoxin LAL (EU/µg) ≤0,1
Biological activity:
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0,1 ng/ml, Bioactivity ≥1,0×10⁷ units/mg
Gene ID:
P06804
Reconstitution instructions:
Sterile water at 0,1 mg/ml
Endotoxin-free:
N
Carrier-free:
Y
Protease-free:
N
Animal-free:
N
Protein synonyms:
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/peptide name:
TNF-A
Purity:
≥95%
Molecular weight:
17,4 kDa
Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Endotoxin level:
Low
Formulation:
10 mM sodium phosphate, 50 mM sodium chloride, pH 7,5
Nuclease-free:
N
Grade:
RUO
Shipping temperature:
RT
Tested applications:
Bioassay