Normal Human Epidermal Melanocytes (NHEM)

Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs.

• Available from juvenile foreskin or adult skin from different locations
• Available from light moderate and dark skin tones
• Donor hair and eye color available on request
• Mel-5 positive
• Cultured in PMA containing or PMA-free Medium

The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.

PromoCell melanocytes are isolated using either the serum-free, PMA (Phorbol Myristate Acetate)-containing Melanocyte Growth Medium or the serum-free and PMA-free Melanocyte Growth Medium M2. Since PMA is a tumour promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M2 and also using this medium for cultivation.

Applications include: Dermatological research, melanoma research, Melanogenesis studies, cosmetic and toxicological testing, skin compound screening.

Ordering information: Human Epidermal Melanocytes can be ordered as cryopreserved or proliferating cells and from juvenile and adult donors. Cell pellets stored in RNAlater® are also available.