The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.

• Simultaneous staining of both the nucleolus and the nucleus in live cells
• High resistance to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods
• Validated for utility in live cell imaging applications, demonstrating appropriate response to treatment with well characterized nucleolus-perturbation agents
• Stringently manufactured, to control and eliminate non-specific assay artifacts

The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.

A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.