Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.

Recognizes human LC3. Recognizes both forms of endogenous LC3. Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot.

For Immunocytochemistry, Immunohistochemistry, Western Blot

Type: Primary
Antigen: LC3
Clonality: Monoclonal
Clone:
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human