Specifications

Pk:
50 µG
Enzyme type:
Recombinant
Source:
E. coli
Species:
M. tuberculosis
Tag sequence:
His Tag
Storage conditions:
Stable for at least 6 months after receipt when stored at –80 °C.
Enzyme name:
NAD Synthetase
Enzyme activity:
~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1umol NAD per min.
Purity:
>98% (SDS-PAGE)
Formulation:
Liquid, In 20 mM TRIS-HCl, pH 7.8.

Specifications

About this item

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalysing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H₂O = AMP + diphosphate + NAD+ + L-glutamate.

Fusion-Tag: His Tag

This recombinant proteins is for research use only.