Enhanced chemifluorescent HRP substrate kit, QuantaRed™
PIER15159
:
- Pk:1
- Description:QuantaRed™ Enhanced chemifluorescent HRP substrate kit, sufficient for 960 wells (10×96-well plates) at 100 µl working volume
QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.
- Low picogram to femtogram detection
- Minimal autofluorescence interference from biological samples
- Use Stop Solution to effectively stop the reaction resulting in stable signal for at least 4 hours
- Produces a strong colorimetric signal and can be read fluorescently or colorimetrically at 570 nm
ADHP is a non-fluorescent compound that reacts with horseradish peroxidase (HRP) to produce resorufin, a soluble, highly fluorescent reaction product with excitation/emission maxima of ~570/585nm. The long wavelength emission of the resorufin minimises interference from the low wavelength (blue and green) autofluorescence, making it an ideal substrate for quantitating target molecules in biological samples.
: Kit includes QuantaRed™ ADHP Concentrate (1 ml), QuantaRed™ Enhancer Solution (50 ml), QuantaRed™ Stable Peroxide Solution (50 ml), QuantaRed™ Stop Solution (10 ml).