Small-scale SEC columns, Superose 6 Increase

Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.

• Wide fractionation range: Optimized to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments
• Very high resolution: Small bead size and a narrow particle size distribution provide high resolution, for high protein purity
• High flow rates: Rigid beads give excellent pressure/flow properties
• Enhanced performance: Improved resolution and runtime compared to predecessor

Superose 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose 6 Increase columns deliver higher resolution protein purification in shorter runtimes.

Suitable for both preparative and analytical applications, Superose 6 Increase SEC columns are the first choice for the characterization of large proteins and protein complexes. They can also be used the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.

Superose 6 Increase is designed for separation of protein complexes and other very large molecules, including those with molecular weights exceeding 400 kD. The optimal fractionation range of Superdex 200 Increase is complementary, providing higher resolution for proteins with molecular weights below 400 kD.

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.