You searched for: Microscopy and staining reagents

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: PanReac AppliChem

Biochemical grade immersion oil for microscopy.

Supplier: ENZO LIFE SCIENCES

Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.

Supplier: Merck

Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: Biotium

CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

Supplier: Biotium

PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

Supplier: ENZO LIFE SCIENCES

The IHC PAP pen provides a hydrophobic barrier when a circle is drawn around a specimen on a glass slide.

Supplier: ENZO LIFE SCIENCES

Fluoromount is an aqueous-based permanent, high stability mounting medium designed for fluorogenic labelling and ideal for long-term storage of processed tissue.

Supplier: ENZO LIFE SCIENCES

IHC mount is an aqueous-based permanent, high stability mounting medium designed for use with chromogenic labeling. Use for mounting of tissue specimens stained with peroxidase and alkaline phosphatase-based systems and various fluorescent dyes.

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

Supplier: Merck

Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.

Supplier: PanReac AppliChem

This fluorescence-free immersion oil for microscopy is highly UV-permeable and free of PCB's.

Supplier: Thermo Fisher Scientific

Pierce silver stain rescue reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.

Supplier: AAT BIOQUEST

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: AAT BIOQUEST

When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50 to 70%.

Supplier: AAT BIOQUEST

The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

Supplier: PanReac AppliChem

Davidson solution is a rapid fixative.

Supplier: PanReac AppliChem

Fixative for preserving soft and delicate structures, used as a mordant in various trichrome procedures.

Supplier: AAT BIOQUEST

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: AAT BIOQUEST

FluoroQuest™ Antifade mounting medium is a specialised and stable formula that effectively preserves fluorescence by preventing rapid photobleaching of fluorescent proteins and dyes.

Supplier: AAT BIOQUEST

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: AAT BIOQUEST

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: AAT BIOQUEST

FluoroQuest™ PLUS Antifade mounting medium, the latest breakthrough in non-setting media technology, provides unparalleled protection against fading throughout the visible spectrum, including far-red wavelengths.

Supplier: AAT BIOQUEST

FluoroQuest™ TSA/PSA Antifade mounting medium is the only mounting medium that is optimised for tyramide (TSA) and styramide (PSA)-based fluorescence imaging applications.

Supplier: AAT BIOQUEST

Nucleolus Red™ DCS1 is a cell-impermeant fluorescent dye that selectively binds to RNA within the nucleolus, a non-membrane-bound nuclear compartment responsible for ribosomal RNA (rRNA) transcription and processing. Upon binding to RNA, the dye exhibits strong red fluorescence, whereas its interaction with DNA results in only weak fluorescence, providing exceptional specificity for nucleolar RNA detection.

Supplier: AAT BIOQUEST

Nucleolus Blue™ DCS1 is a cell-impermeant fluorescent dye that selectively binds to RNA within the nucleolus, a non-membrane-bound nuclear compartment responsible for ribosomal RNA (rRNA) transcription and processing. Upon binding to RNA, the dye exhibits strong blue fluorescence, whereas its interaction with DNA results in only weak fluorescence, providing exceptional specificity for nucleolar RNA detection.

Supplier: AAT BIOQUEST

Nucleolus Green™ LCS1 is a cell-permeant fluorescent dye that selectively binds to RNA within the nucleolus, a non-membrane-bound nuclear compartment responsible for ribosomal RNA (rRNA) transcription and processing. Upon binding to RNA, the dye exhibits strong green fluorescence, whereas its interaction with DNA results in only weak fluorescence, providing exceptional specificity for nucleolar RNA detection.

Supplier: GLASWARENFABRIK KARL HECHT

Mounting medium for histology/microscopy.