You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
DNA marker, broad range
Supplier: Thermo Fisher Scientific
From 250 to 5000 base pairs: 5 bands from 125 to 1000 bp; four bands from 1500 to 5000 bp. Recommended load volume 20 µl.
Expand 2 Items
DNA marker, high range, 1000 bp ladder
Supplier: Thermo Fisher Scientific
DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. They are commonly used in the studies of plasmid DNA and PCR products.
Expand 2 Items
PCR Molecular Size Marker
Supplier: Merck Millipore (Novagen)
PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.
Expand 1 Items
Western blotting combination pack, Amersham ECL Plex™
Supplier: Cytiva
Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.
Expand 2 Items
Western blotting system, Amersham ECL™
Supplier: Cytiva
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
Expand 1 Items
Amersham™ ECL Direct™ Detection Reagents, Cytiva
Supplier: Cytiva
Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.
Expand 2 Items
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva
Supplier: Cytiva
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.
Expand 2 Items
Antibody diluent, MP Biomedicals
Supplier: MP Biomedicals
This antibody diluent is compatible with common stabilising compounds at pH 7,3.
Expand 1 Items
Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia
Supplier: Cytiva
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Expand 4 Items
Cy™ fluorescent amidites
Supplier: Cytiva
Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.
Expand 3 Items
Western blotting detection reagent, Amersham™ ECL™ start
Supplier: Cytiva
Chemiluminescence detection reagent for detection of high to medium abundant proteins.
Expand 2 Items
Western blotting blocking reagent, Amersham ECL™ Prime
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
Expand 1 Items
Amersham ECL Direct Labeling and Detection System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Expand 2 Items
Perfect DNA™ ladders
Supplier: Merck Millipore (Novagen)
The Perfect DNA™ ladders contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities and cover a wide range of DNA sizes.
Expand 5 Items
DNA QuantLadders
Supplier: LONZA
QuantLadders are prepared from five highly purified DNA fragments that provide visual quantitation of DNA within ±1% accuracy. The DNA QuantLadder provides each DNA fragment in perfect relative mass equivalence at 100 ng, 70 ng, 50 ng, 20 ng and 10 ng. The reverse QuantLadder simply reverses the mass equivalence order of the QuantLadder.
Expand 1 Items
DNA ladder, 100 bp
Supplier: PanReac AppliChem
This DNA ladder is ideal for determining the size of double-stranded DNA ranging from 100 to 1,000 base pairs. The ladder consists of ten fragments that range in size from 100 to 1,000 bp in 100 bp increments. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.
Expand 1 Items
Substrate for Western blotting, ECF™
Supplier: Cytiva
ECF™ substrate for Western blotting
Expand 1 Items
Amersham ECL Direct Labeling System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Expand 1 Items
pI markers
Supplier: Cytiva
pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.
Expand 3 Items
Protein and peptide molecular weight markers
Supplier: Cytiva
For determination of molecular weight of uncharacterised protein samples with precision by comparison with carefully selected standards.
Expand 2 Items
Molecular weight markers, Rainbow™
Supplier: Cytiva
Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.
Expand 3 Items
Western blotting markers, Amersham™ ECL™ DualVue™
Supplier: Cytiva
Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.
Expand 1 Items
Protein standards
Supplier: Genscript
GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards.
Expand 5 Items
Molecular weight markers, Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers
Supplier: Cytiva
Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers are protein molecular weight markers optimised for use with the ECL™ Plex™ Western blotting detection system. They are designed to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels.
Expand 2 Items
BCIP®/NBT alkaline phosphatase substrate, SIGMAFAST™
Supplier: Merck
SIGMAFAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity.
Expand 2 Items
IEF carrier ampholytes, SERVALYT™
Supplier: Serva
IEF carrier ampholyte, SERVALYT™, pH 3 - 10, analytical grade
Expand 46 Items
DNA Ladder 1 kb
Supplier: STEMCELL Technologies
DNA ladder consisting of 13 fragments from 250 to 10000 base pairs for determining the size of double-stranded DNA by gel electrophoresis.