You searched for: Electrophoresis Reagents

Supplier: Thermo Fisher Scientific

MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.

Supplier: Thermo Fisher Scientific

RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

Supplier: Rockland Immunochemicals

Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III.

Supplier: Thermo Fisher Scientific

O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.

Supplier: Thermo Fisher Scientific

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Supplier: ENZO LIFE SCIENCES

AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

Supplier: ENZO LIFE SCIENCES

AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp, 1500 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ GeneRuler™ and O'GeneRuler™ DNA ladders are mixtures of chromatography-purified individual DNA fragments. The GeneRuler™ line includes the most popular ladders, such as the 100 bp and 1 kb DNA Ladder. GeneRuler™ DNA ladders are available in two formats: Conventional (provided in TE buffer) and a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF). O'GeneRuler™ DNA ladders are another ready-to-use version of GeneRuler™ DNA ladders that are premixed with 6X Orange DNA Loading Dye.

Supplier: ENZO LIFE SCIENCES

AMPIGENE® DNA ladder contains DNA fragments ranging from 250 to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 1000 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

Supplier: Thermo Fisher Scientific

pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.

Supplier: MP Biomedicals

The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.

Supplier: Thermo Fisher Scientific

The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

Supplier: Thermo Fisher Scientific

LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

Supplier: Thermo Fisher Scientific

Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

Supplier: Thermo Fisher Scientific

The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

Supplier: AAT BIOQUEST

Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

Supplier: Thermo Fisher Scientific

The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and in situ hybridisation applications.

Supplier: Thermo Fisher Scientific

Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.

Supplier: Thermo Fisher Scientific

QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.

Supplier: Thermo Fisher Scientific

The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and in situ hybridisation applications.

Supplier: G-Biosciences

High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.

Supplier: Thermo Fisher Scientific

QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.

Supplier: G-Biosciences

G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.

Supplier: Thermo Fisher Scientific

The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.

Supplier: Thermo Fisher Scientific

This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

Supplier: Thermo Fisher Scientific

Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.

Supplier: Thermo Fisher Scientific

SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.

Supplier: Thermo Fisher Scientific

Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Supplier: Thermo Fisher Scientific

SuperSignal® ELISA Femto maximum sensitivity substrate utilises the SuperSignal® system with an enhancer formulation for superior protein detection and low-end linearity in ELISA applications. Ideal for automated high-throughput screening applications.

Supplier: Thermo Fisher Scientific

LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).