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You searched for: Electrophoresis Reagents

Electrophoresis Reagents

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Peqlab peqGOLD, DNA Ladder, 100 bp

Peqlab peqGOLD, DNA Ladder, 100 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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Peqlab peqGOLD, DNA Ladders, 50 bp

Peqlab peqGOLD, DNA Ladders, 50 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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Peqlab peqGOLD, Protein Marker I (Unstained)

Peqlab peqGOLD, Protein Marker I (Unstained)

Supplier: VWR Chemicals

peqGOLD Protein Marker I is a mixture of seven purified proteins which are re-dissolved 'ready to use' in loading buffer. The proteins resolve into sharp, tight bands in the range of 14,4 to 116,0 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250.

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VWR®, Protein marker VI (pre-stained), peqGOLD

VWR®, Protein marker VI (pre-stained), peqGOLD

Supplier: VWR Chemicals

peqGOLD pre-stained Protein Marker VI is a mixture of 10 coloured proteins. These proteins are recombinantly produced in E.coli, highly purified and supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS polyacrylamide gels.

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VWR®, DNA ladders

VWR®, DNA ladders

Supplier: VWR Chemicals

VWR® DNA ladders are convenient dsDNA ladders supplied in a loading buffer, which are ready to use on agarose and SDS DNA gels.

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VWR® Ready Ladder™ DNA Ladder, 50 bp

VWR® Ready Ladder™ DNA Ladder, 50 bp

Supplier: VWR Chemicals

Ladder with 10 fragments ranging from 50 to 500 bp for easy band identification. Ready to use formula pre-mixed with loading dye.

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Peqlab peqGOLD, DNA Ladder Mix

Peqlab peqGOLD, DNA Ladder Mix

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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VWR®, DNA marker, 1 kb

VWR®, DNA marker, 1 kb

Supplier: VWR Chemicals

DNA marker consists of 11 fragments ranging from 500 to 10000 bp for easy band identification.

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Peqlab peqGOLD, DNA Ladder, 1 kb

Peqlab peqGOLD, DNA Ladder, 1 kb

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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VWR®, DNA ladder, 100 bp

VWR®, DNA ladder, 100 bp

Supplier: VWR Chemicals

Ladder with 13 fragments ranging from 100 to 3000 bp for easy band identification.

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VWR® EZ-Vision®, DNA Ladder, 100 bp

VWR® EZ-Vision®, DNA Ladder, 100 bp

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

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Western blot enhancer, SuperSignal™, Pierce

Western blot enhancer, SuperSignal™, Pierce

Supplier: Thermo Fisher Scientific

SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

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S-Nitrosylation western blot kit, Pierce™

S-Nitrosylation western blot kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

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Fluorescent western blot stripping buffer, Restore™

Fluorescent western blot stripping buffer, Restore™

Supplier: Thermo Fisher Scientific

Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

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DNA ladder 50 to 1500 bp, AMPIGENE®

Supplier: ENZO LIFE SCIENCES

AMPIGENE® DNA ladder contains DNA fragments ranging from 50 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 200 bp, 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

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Upstate® Autorad Orientation Markers for Western Blotting

Upstate® Autorad Orientation Markers for Western Blotting

Supplier: Merck

Autorad orientation markers for use on immunoblots in detection of Chemiluminescence in Western blotting, dot/slot blotting.

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Protein ladders (pre-stained), ScanLater™

Protein ladders (pre-stained), ScanLater™

Supplier: Molecular Devices

The ScanLater™ Western blot protein ladder is a mixture of seven biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD.

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DNA ladders

Supplier: Biotium

Ready to use DNA ladders are supplied in 1X loading buffer at an optimal concentration for loading GelRed™ and GelGreen™ precast gels.

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Western blot stripping buffer, restore PLUS™

Western blot stripping buffer, restore PLUS™

Supplier: Thermo Fisher Scientific

Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

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Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate

Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate

Supplier: Thermo Fisher Scientific

SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

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Substrate for western blotting, NBT (nitro-blue tetrazolium chloride)

Substrate for western blotting, NBT (nitro-blue tetrazolium chloride)

Supplier: Thermo Fisher Scientific

NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

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Western blotting kit, ECL substrate, Pierce™

Western blotting kit, ECL substrate, Pierce™

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

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Peroxidase conjugate stabiliser/diluent, Guardian™

Peroxidase conjugate stabiliser/diluent, Guardian™

Supplier: Thermo Fisher Scientific

Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

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Pierce™ Clean-Blot™ IP Detection Kit and Reagents

Pierce™ Clean-Blot™ IP Detection Kit and Reagents

Supplier: Thermo Fisher Scientific

Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.

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Horseradish peroxidase, Pierce™

Horseradish peroxidase, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

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DAB substrate

DAB substrate

Supplier: Thermo Fisher Scientific

DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.

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Biotin random prime DNA labelling kit, North2South™

Biotin random prime DNA labelling kit, North2South™

Supplier: Thermo Fisher Scientific

North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.

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HRP conjugates, HisProbe™

HRP conjugates, HisProbe™

Supplier: Thermo Fisher Scientific

HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

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DNA ladder 250 to 10000 bp, AMPIGENE®

Supplier: ENZO LIFE SCIENCES

AMPIGENE® DNA ladder contains DNA fragments ranging from 250 to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 1000 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

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DNA marker, pBR322 DNA/BsuRI (HaeIII) marker

DNA marker, pBR322 DNA/BsuRI (HaeIII) marker

Supplier: Thermo Fisher Scientific

pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.

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