You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Peqlab peqGOLD, DNA Ladder, 100 bp
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
Expand 1 Items
Peqlab peqGOLD, DNA Ladders, 50 bp
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
Expand 1 Items
Peqlab peqGOLD, Protein Marker I (Unstained)
Supplier: VWR Chemicals
peqGOLD Protein Marker I is a mixture of seven purified proteins which are re-dissolved 'ready to use' in loading buffer. The proteins resolve into sharp, tight bands in the range of 14,4 to 116,0 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250.
Expand 1 Items
VWR®, Protein marker VI (pre-stained), peqGOLD
Supplier: VWR Chemicals
peqGOLD pre-stained Protein Marker VI is a mixture of 10 coloured proteins. These proteins are recombinantly produced in E.coli, highly purified and supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS polyacrylamide gels.
Expand 1 Items
VWR®, DNA ladders
Supplier: VWR Chemicals
VWR® DNA ladders are convenient dsDNA ladders supplied in a loading buffer, which are ready to use on agarose and SDS DNA gels.
Expand 3 Items
VWR® Ready Ladder™ DNA Ladder, 50 bp
Supplier: VWR Chemicals
Ladder with 10 fragments ranging from 50 to 500 bp for easy band identification. Ready to use formula pre-mixed with loading dye.
Expand 1 Items
Peqlab peqGOLD, DNA Ladder Mix
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
Expand 1 Items
VWR®, DNA marker, 1 kb
Supplier: VWR Chemicals
DNA marker consists of 11 fragments ranging from 500 to 10000 bp for easy band identification.
Expand 1 Items
Peqlab peqGOLD, DNA Ladder, 1 kb
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
Expand 1 Items
VWR®, DNA ladder, 100 bp
Supplier: VWR Chemicals
Ladder with 13 fragments ranging from 100 to 3000 bp for easy band identification.
Expand 1 Items
VWR® EZ-Vision®, DNA Ladder, 100 bp
Supplier: VWR Chemicals
EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.
Expand 1 Items
Western blot enhancer, SuperSignal™, Pierce
Supplier: Thermo Fisher Scientific
SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.
Expand 2 Items
S-Nitrosylation western blot kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.
Expand 2 Items
Fluorescent western blot stripping buffer, Restore™
Supplier: Thermo Fisher Scientific
Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.
Expand 2 Items
DNA ladder 50 to 1500 bp, AMPIGENE®
Supplier: ENZO LIFE SCIENCES
AMPIGENE® DNA ladder contains DNA fragments ranging from 50 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 200 bp, 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
Expand 1 Items
Upstate® Autorad Orientation Markers for Western Blotting
Supplier: Merck
Autorad orientation markers for use on immunoblots in detection of Chemiluminescence in Western blotting, dot/slot blotting.
Expand 1 Items
Protein ladders (pre-stained), ScanLater™
Supplier: Molecular Devices
The ScanLater™ Western blot protein ladder is a mixture of seven biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD.
Expand 1 Items
DNA ladders
Supplier: Biotium
Ready to use DNA ladders are supplied in 1X loading buffer at an optimal concentration for loading GelRed™ and GelGreen™ precast gels.
Expand 1 Items
Western blot stripping buffer, restore PLUS™
Supplier: Thermo Fisher Scientific
Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.
Expand 2 Items
Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate
Supplier: Thermo Fisher Scientific
SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.
Expand 2 Items
Substrate for western blotting, NBT (nitro-blue tetrazolium chloride)
Supplier: Thermo Fisher Scientific
NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.
Expand 1 Items
Western blotting kit, ECL substrate, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.
Expand 2 Items
Peroxidase conjugate stabiliser/diluent, Guardian™
Supplier: Thermo Fisher Scientific
Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.
Expand 2 Items
Pierce™ Clean-Blot™ IP Detection Kit and Reagents
Supplier: Thermo Fisher Scientific
Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.
Expand 2 Items
Horseradish peroxidase, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
Expand 1 Items
DAB substrate
Supplier: Thermo Fisher Scientific
DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
Expand 1 Items
Biotin random prime DNA labelling kit, North2South™
Supplier: Thermo Fisher Scientific
North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
Expand 1 Items
HRP conjugates, HisProbe™
Supplier: Thermo Fisher Scientific
HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
Expand 1 Items
DNA ladder 250 to 10000 bp, AMPIGENE®
Supplier: ENZO LIFE SCIENCES
AMPIGENE® DNA ladder contains DNA fragments ranging from 250 to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 1000 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
Expand 1 Items
DNA marker, pBR322 DNA/BsuRI (HaeIII) marker
Supplier: Thermo Fisher Scientific
pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.