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You searched for: Electrophoresis Reagents

Electrophoresis Reagents

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Heart tissue region blots, single species

Heart tissue region blots, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.

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Mouse single tissue blot, single species

Mouse single tissue blot, single species

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Rat single tissue blot, single species

Rat single tissue blot, single species

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X

Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Visualizer and Visualizer EC Western Blot Detection Kits

Visualizer and Visualizer EC Western Blot Detection Kits

Supplier: Merck

The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.

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Chemicon® ReBlot Western Blot Recycling Kit

Chemicon® ReBlot Western Blot Recycling Kit

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Chemiluminescent detection reagents, Immobilon® Western HRP substrates

Chemiluminescent detection reagents, Immobilon® Western HRP substrates

Supplier: Merck

Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

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Single tissue blots, multiple species

Single tissue blots, multiple species

Supplier: G-Biosciences

Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.

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Diluent for western blotting, Swift™ Western Diluent

Diluent for western blotting, Swift™ Western Diluent

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

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Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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NPP ELISA alkaline phosphatase substrate

Supplier: Rockland Immunochemicals

NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

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Transfer pads for western blotting, Swift™

Transfer pads for western blotting, Swift™

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

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Western blots, pre-made, human single tissue single species

Western blots, pre-made, human single tissue single species

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

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Botanical garden blot

Botanical garden blot

Supplier: G-Biosciences

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

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Caspase-3 substrates, NucView®

Caspase-3 substrates, NucView®

Supplier: Biotium

NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

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Chemiluminescent detection reagents, Luminata™ Western HRP substrates

Chemiluminescent detection reagents, Luminata™ Western HRP substrates

Supplier: Merck

Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.

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Normal cell line blots, human

Normal cell line blots, human

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

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CYTAG™ CGH labelling kits

CYTAG™ CGH labelling kits

Supplier: ENZO LIFE SCIENCES

The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.

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IHC chromogen substrate (AEC, stable), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.

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Peqlab peqGOLD, Protein Marker V (Pre-Stained)

Peqlab peqGOLD, Protein Marker V (Pre-Stained)

Supplier: VWR Chemicals

Protein Marker V (pre-stained) for monitoring protein separation, transfer efficiency and size determination.

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VWR® EZ-Vision®, DNA Ladder, 1 kb

VWR® EZ-Vision®, DNA Ladder, 1 kb

Supplier: VWR Chemicals

EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

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Peqlab peqGOLD, Protein Marker II (Unstained)

Peqlab peqGOLD, Protein Marker II (Unstained)

Supplier: VWR Chemicals

Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.

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VWR® RiboReady™ Color Micro RNA Ladder

VWR® RiboReady™ Color Micro RNA Ladder

Supplier: VWR Chemicals

RiboReady™ Color Micro RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels. The apparent sizes of bands in the RiboReady™ Color Micro RNA ladder are 100, 75, 50, 40, 30 and 20 bases in length.

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Peqlab peqGOLD, DNA Ladders, 50 bp

Peqlab peqGOLD, DNA Ladders, 50 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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Peqlab peqGOLD, DNA Ladder, 100 bp

Peqlab peqGOLD, DNA Ladder, 100 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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Peqlab peqGOLD, DNA Ladder, 100 bp Plus

Peqlab peqGOLD, DNA Ladder, 100 bp Plus

Supplier: VWR Chemicals

peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.

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Peqlab peqGOLD, Protein Marker IV (Pre-Stained)

Peqlab peqGOLD, Protein Marker IV (Pre-Stained)

Supplier: VWR Chemicals

peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.

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VWR® PCR DNA Marker

VWR® PCR DNA Marker

Supplier: VWR Chemicals

PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

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VWR®, DNA ladders

VWR®, DNA ladders

Supplier: VWR Chemicals

VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

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VWR® RiboReady™, Color RNA Ladder, 1 kb

VWR® RiboReady™, Color RNA Ladder, 1 kb

Supplier: VWR Chemicals

RiboReady™ Color 1 kb RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels.

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