You searched for: Nucleic Acid Reagents

Supplier: Oxford Nanopore Technologies

Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

Supplier: Oxford Nanopore Technologies

Additional flow cell priming reagents to enable additional loading of more library during a sequencing run.

Supplier: Quantabio

sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Supplier: Oxford Nanopore Technologies

PCR Barcoding Expansion packs enable multiple nanopore sequencing libraries to be pooled and run on a single MinION Flow Cell.

Supplier: Oxford Nanopore Technologies

The cDNA-PCR Sequencing Kits enable the identification and quantification of full-length transcripts with highest output using nanopore sequencing.

Supplier: Oxford Nanopore Technologies

The 16S Barcoding Kit delivers rapid, genus-level bacteria identification using nanopore sequencing.

Supplier: Oxford Nanopore Technologies

Rapid Sequencing Kits offer a simple and rapid library preparation method for genomic DNA sequencing on MinION™ devices.

Supplier: Oxford Nanopore Technologies

Additional extraction EB (EEB)

Supplier: Oxford Nanopore Technologies

An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).

Supplier: Oxford Nanopore Technologies

A sequencing kit optimised for sequencing native RNA with improved output and accuracy.

Supplier: Oxford Nanopore Technologies

DNA or RNA for sequencing control experiments.

Supplier: Oxford Nanopore Technologies

An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.

Supplier: Oxford Nanopore Technologies

An expansion pack designed for size selection of high molecular weight DNA samples to progressively deplete short fragments of <25 kb.

Supplier: Biotium

The AccuBlue® NextGen dsDNA Quantitation Kit offers unprecedented level of sensitivity in dsDNA detection. It is a sensitive and accurate DNA quantitation method for ultra-low amounts of DNA with a detection limit as low as one pg DNA in a 96-well plate reader assay.

Supplier: Oxford Nanopore Technologies

This expansion is recommended for users who want to make use of unused barcodes to maximise the use of their Native Barcoding Kits.

Supplier: Oxford Nanopore Technologies

Additional sequencing buffer, elution buffer, and loading beads.

Supplier: MP Biomedicals

The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.

Supplier: MP Biomedicals

Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.

Supplier: AGILENT

The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

Supplier: AGILENT

Protein directed evolution in just one step.

Supplier: AGILENT

For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Supplier: AGILENT

The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Supplier: AGILENT

The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

Supplier: Thermo Fisher Scientific

The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Supplier: AGILENT

Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.