You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric Glucose Quantitation Kit
Supplier: AAT BIOQUEST
Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit
Supplier: AAT BIOQUEST
Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.
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Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit
Supplier: AAT BIOQUEST
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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Amplite® Fluorimetric Alanine Aminotransferase Assay Kit
Supplier: AAT BIOQUEST
Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.
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Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*
Supplier: AAT BIOQUEST
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® Ethanol Quantitation Kit
Supplier: AAT BIOQUEST
The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.
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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT BIOQUEST
Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Formaldehyde is a naturally occurring substance.
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Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*
Supplier: AAT BIOQUEST
Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.
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Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
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Amplite® Colorimetric Total NAD and NADH Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric NAD/NADH Ratio Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Yeast β-galactosidase assay kit, Pierce™
Supplier: Thermo Fisher Scientific
Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.
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Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.
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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Amplite® Fluorimetric Aldehyde Quantitation Kit
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT BIOQUEST
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit
Supplier: AAT BIOQUEST
Site-selective modification of proteins is of major interest in chemical biology.
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Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.
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Amplite® Fluorimetric Mercuric Ion Quantitation Kit
Supplier: AAT BIOQUEST
Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.
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Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.
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Amplite® Choline Quantitation Kit
Supplier: AAT BIOQUEST
Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).
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Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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ReadiUse™ NADP Regenerating Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.
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Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Serine hydrolase probes, ActivX™ FP
Supplier: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.