You searched for: Cellular Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
DuraClone Dry Unitized Antibody Panels For Flow Cytometry
Supplier: Beckman Coulter
The dry DURAClone antibody panels are designed for clinical research studies. Every assay tube or well contains a layer with the dried-down antibody panel unitized for a single test.
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DNMT colorimetric drug discovery tests
Supplier: ENZO LIFE SCIENCES
DNMT colorimetric drug discovery kit is a simple method to detect DNA methylation activity or inhibition.
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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Apoptosis assay kit (Dual), NucView™ 488
Supplier: Biotium
NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.
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FLUOR DE LYS® HDAC3/NCOR1 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Nuclear-ID™ red cell cycle kit, GFP-CERTIFIED®, for flow cytometry
Supplier: ENZO LIFE SCIENCES
GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry.
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Red hydrogen peroxide assay kit
Supplier: ENZO LIFE SCIENCES
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.
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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.
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Cell proliferation assay kit, WST-1
Supplier: Cayman Chemical
WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.
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Green long-term cell tracer, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.
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CV-Cathepsin L detection kit
Supplier: ENZO LIFE SCIENCES
Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.
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FLUOR DE LYS® SIRT3 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT3 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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MaxSpec® Dienes Derivatization Kit
Supplier: Cayman Chemical
Derivatization Kit for the sensitive LC-MS/MS analysis of dienes such as Vitamin D and Vitamin D metabolites.
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ApoSENSOR™ Cell viability assay kit
Supplier: ENZO LIFE SCIENCES
The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
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Calcium assay kits, FLIPR® Calcium 4 assay kit
Supplier: Molecular Devices
The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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Cardiotoxicity kit, EarlyTox™
Supplier: Molecular Devices
The EarlyTox™ Cardiotoxicity Kit provides a fast, simple and reliable fluorescence based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes.
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Nitrotyrosine IP Kit
Supplier: Cayman Chemical
For the capture and pulldown of nitrated proteins from cell and tissue lysates.
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FLUOR DE LYS® HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.
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ROS-ID™ NO Detection kit
Supplier: ENZO LIFE SCIENCES
The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.
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CF® Dye Dextran 40000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 40,000 MW, and contains a formaldehyde-fixable free-amine group.
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Annexin V CF® dye conjugates, azide-free, lyophilised
Supplier: Biotium
Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments, fluorescence microscopy, or flow cytometry.
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NF[kappa]B transcription factor assay kits
Supplier: Rockland Immunochemicals
These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.
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Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.
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CF® Dye Dextran 150000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.
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ER-ID™ green assay kit
Supplier: ENZO LIFE SCIENCES
The ER-ID® Green assay kit is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
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Cell integrity kit, EarlyTox™
Supplier: Molecular Devices
The EarlyTox™ Cell Integrity Kit enables the differentiation of live cells from dead cells via fluorescent labeling. This is useful for the rapid quantification of cell viability when used with the SpectraMax® MiniMax™ 300 Imaging Cytometer or other fluorescence-based cellular imaging instruments.
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Cell viability assay kits, EarlyTox™ live/dead assay kits
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Reporter assay kits, SpectraMax® Glo Steady-Luc™
Supplier: Molecular Devices
The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.
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Ubiquitin activating kit
Supplier: ENZO LIFE SCIENCES
Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.
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Cyclic-AMP fluorescent assay kits, CatchPoint™
Supplier: Molecular Devices
CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.