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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Big blue

Supplier: AGILENT

These are used in all Big Blue rodents and cell lines.

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Green detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

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ROS-ID™ total ROS/superoxide detection kit

ROS-ID™ total ROS/superoxide detection kit

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.

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Cyclic-GMP fluorescent assay kits, CatchPoint™

Cyclic-GMP fluorescent assay kits, CatchPoint™

Supplier: Molecular Devices

CatchPoint™ cGMP Fluorescent Assay Kits measure cGMP levels via a competitive immunoassay format. The second messenger cyclic GMP (cGMP) modulates cellular processes, including smooth muscle relaxation, kidney function, and inflammation, by activating effectors such as protein kinases, cGMP-dependent phosphodiesterases, and cGMP-gated ion channels.

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Calcium assay kits, FLIPR® Calcium 5 assay kit

Calcium assay kits, FLIPR® Calcium 5 assay kit

Supplier: Molecular Devices

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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Comet SCGE assay kit

Supplier: ENZO LIFE SCIENCES

COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

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ApoSENSOR™ ADP/ATP ratio assay kit

Supplier: ENZO LIFE SCIENCES

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.

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Calcein-AM cell viability assay kits

Calcein-AM cell viability assay kits

Supplier: Biotium

Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.

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Green detection kit, LYSO-ID®

Supplier: ENZO LIFE SCIENCES

LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

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Red long-term cell tracer, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

CYTO-ID® is a live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

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QBT™ Fatty acid uptake assay kit

QBT™ Fatty acid uptake assay kit

Supplier: Molecular Devices

QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.

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Millicell® µ-Migration Assay Kit

Millicell® µ-Migration Assay Kit

Supplier: Merck

This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours.

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Solutions colorantes, Ghost Dye™

Solutions colorantes, Ghost Dye™

Supplier: Tonbo Biosciences

Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.

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Black IHC chromogen (HRP), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit

Supplier: ENZO LIFE SCIENCES

The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.

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HIF-1α transcription factor assay Kit

HIF-1α transcription factor assay Kit

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

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Glutathione Cell-Based Detection Kit (Blue Fluorescence)

Glutathione Cell-Based Detection Kit (Blue Fluorescence)

Supplier: Cayman Chemical

For rapid and easy determination of GSH in whole cells.

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FLUOR DE LYS® HDAC3/NCOR1 fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

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Acetyltransferase activity kit

Supplier: ENZO LIFE SCIENCES

For the measurement of acetyl-CoA dependent acetyltransferase activity in purified in vitro samples that do not contain reductants from any species.

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Nitric oxide reagents and kits

Nitric oxide reagents and kits

Supplier: Biotium

Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.

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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

Supplier: ENZO LIFE SCIENCES

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

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Glutathione Peroxidase Assay Kit

Glutathione Peroxidase Assay Kit

Supplier: Cayman Chemical

For the measurement of glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

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Catalase fluorometric detection kit

Supplier: ENZO LIFE SCIENCES

The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.

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LEADING LIGHT® Wnt cell line medium pack

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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Prostaglandin Screening Library I

Prostaglandin Screening Library I

Supplier: Cayman Chemical

For screening of PGF analogs.

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Apoptosis assay kit (Dual), NucView™ 488

Apoptosis assay kit (Dual), NucView™ 488

Supplier: Biotium

NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.

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Calcium assay kits, FLIPR® Calcium 4 assay kit

Calcium assay kits, FLIPR® Calcium 4 assay kit

Supplier: Molecular Devices

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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CV-Caspase 3 & 7 Detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

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Prostaglandin Screening Library III

Prostaglandin Screening Library III

Supplier: Cayman Chemical

For A and J series prostaglandin analog screening.

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GOLGI ID® Green assay kit

Supplier: ENZO LIFE SCIENCES

The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.

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