You searched for: Cellular Assays

Supplier: Biotium

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Supplier: ENZO LIFE SCIENCES

Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.

Supplier: ENZO LIFE SCIENCES

Blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.

Supplier: ENZO LIFE SCIENCES

The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

Supplier: ENZO LIFE SCIENCES

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

Supplier: ENZO LIFE SCIENCES

Blue IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: ENZO LIFE SCIENCES

The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.

Supplier: ENZO LIFE SCIENCES

The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.

Supplier: Biotium

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.

Supplier: ENZO LIFE SCIENCES

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

Supplier: ENZO LIFE SCIENCES

Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

Supplier: ENZO LIFE SCIENCES

The set contains five reagents that induce apoptosis through different mechanisms.

Supplier: Cayman Chemical

A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.

Supplier: Biotium

Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.

Supplier: ENZO LIFE SCIENCES

HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

Supplier: G-Biosciences

Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

Supplier: AAT BIOQUEST

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

Supplier: Cayman Chemical

96 well assay for measurement of SREBP-2.

Supplier: ENZO LIFE SCIENCES

Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.