Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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CF® Dye Dextran 150000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.

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APO-BRDU™ kit, TUNEL Assay

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

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CV-Cathepsin K detection kit

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin K LeuArg peptide recognition sequence, this kit allows the detection of cathepsin K activity in living cells by fluorescence microscopy.

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ApoSENSOR™ Cell viability assay kit

Supplier: Enzo Life Sciences

The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).

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RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium

Supplier: Biotium

RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.

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FluoForte™ Reagent

Supplier: Enzo Life Sciences

Calcium dye

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Acetyltransferase activity kit

Supplier: Enzo Life Sciences

For the measurement of acetyl-CoA dependent acetyltransferase activity in purified in vitro samples that do not contain reductants from any species.

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APO-BRDU™ kit, TUNEL Assay

Supplier: Enzo Life Sciences

Cellular assay, APO-BRDU™ kit

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LSD1 fluorometric drug discovery kit

Supplier: Enzo Life Sciences

The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.

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FLUOR DE LYS® HDAC6 fluorometric drug discovery kit

Supplier: Enzo Life Sciences

A Fluor-de-Lys® fluorescent assay system. The HDAC6 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC6 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC6 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.

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Uric acid assay kit

Supplier: Cayman Chemical

Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.

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CYTO-ID® Autophagy detection kit

Supplier: Enzo Life Sciences

The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy and flow cytometry.

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Nitric oxide reagents and kits

Supplier: Biotium

Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.

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Red hydrogen peroxide assay kit

Supplier: Enzo Life Sciences

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.