You searched for: Stains

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: Merck

Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

Supplier: Merck

Neo-Clear® is a non hazardous alternative to xylene. It is virtually odourless, compatible with most high purity solvents and is characterised by lower evaporation rates than xylene, making it a lower health risk.

Supplier: Biotium

Stains specifically designed for DNA in polyacrylamide gels.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.

Supplier: Merck

Kaiser's glycerol gelatin is an aqueous mounting medium for microscopy and works well with specimens prepared for enzyme, lipid, and immunohistochemistry determinations.

Supplier: Biotium

DNAzure® Blue Nucleic Acid Gel Stain is an ultrasensitive reagent for visible staining of dsDNA in agarose or polyacrylamide gels.

Supplier: SIGMA ALDRICH MICROSCOPY

Glycerol alters the hygroscopic property of gelatin and its permeability to water vapour. Glycerol gelatin or glycerine jelly medium is an aqueous slide mounting media, used for mounting samples in microscopy and histology.

Supplier: Merck

Hard tissue like bones or teeth, and all specimens of calcified tissue like bone marrow punches require special pre-treatment before routine processing by a histological laboratory.

Supplier: SIGMA ALDRICH MICROSCOPY

Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.

Supplier: SIGMA ALDRICH MICROSCOPY

Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: G-Biosciences

BLOT-FastStain™ is a unique stain for reversible staining of protein on nitrocellulose and PVDF transfer membranes. It only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility.

Supplier: SIGMA ALDRICH MICROSCOPY

Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

Supplier: Merck

The defined hardness and uniformity of the material to be cut are vital requirements in creating consistent quality in cutting. These requirements can be met using a range of suitable substances, such as paraffins, resins, gelatine and celloidin.

Supplier: G-Biosciences

LabSafe GEL Blue™ is an enhanced protein stain based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualisation.

Supplier: Thermo Fisher Scientific

Oange loading dye (6X) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Ideal for conventional DNA electrophoresis.

Supplier: SIGMA ALDRICH MICROSCOPY

JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: ENZO LIFE SCIENCES

Nucleic acid dye for gel staining and higher sensitivity qPCR.

Supplier: Merck

Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.

Supplier: ENZO LIFE SCIENCES

Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.

Supplier: Biotium

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.

Supplier: Biotium

CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

Supplier: Thermo Fisher Scientific

This gel loading dye contains two different dyes bromophenol blue and xylene cyanol FF for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases.