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Stains

Overcome normal optical limitations in microscopic studies by highlighting specific structures with stains and reagents and then protect samples with fixatives. The fast-acting dyes create strong contrasts between cell components for easier detection or analyses. Sensitive attractions to certain organelle or cellular components differ among offered stains, allowing precise testing modifications. Flexible in use, ready-to-use stains may be handled manually or in conjunction with automatic equipment.

VWR® BANDit™ Protein Stain Blue

VWR® BANDit™ Protein Stain Blue

Supplier: VWR Chemicals

Blue BANDit is a safe and environmentally friendly alternative to traditional Coomassie® Blue staining.

    
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VWR® VividPro™ Plus Fluorescent Protein Stains

VWR® VividPro™ Plus Fluorescent Protein Stains

Supplier: VWR Chemicals

VividPro Plus™ is a fluorescent protein stain that can be used in a fast co-electrophoretic protocol to visualize proteins in SDS PAGE gels.

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VWR® VividPro™ Fluorescent Stains

VWR® VividPro™ Fluorescent Stains

Supplier: VWR Chemicals

VividPro™ is a generic fluorescent stain for protein gel electrophoresis that can be used to visualize proteins.

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Peqlab peqGREEN, DNA/RNA Dye

Peqlab peqGREEN, DNA/RNA Dye

Supplier: VWR Peqlab

peqGREEN dye is a safe alternative to ethidium bromide for staining of dsDNA, ssDNA and RNA.

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VWR® EZ-Vision®, DNA dyes

VWR® EZ-Vision®, DNA dyes

Supplier: VWR Chemicals

EZ-Vision DNA dye as loading buffer provides a non mutagenic, environmentally friendly alternative to ethidium bromide for instantaneous fluorescent DNA band visualisation.

    
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VWR® Gold-N-Gel™, RNA dye

VWR® Gold-N-Gel™, RNA dye

Supplier: VWR Chemicals

Gold-N-Gel RNA dye is compatible with standard protocols for denaturing electrophoresis of RNA fragments. Bands are immediately visible upon illumination with UV light. A standard ethidium bromide filter can be used for gel documentation.

    
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VWR®, DNA loading buffers

VWR®, DNA loading buffers

Supplier: VWR Chemicals

These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.

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VWR® EZ-Vision® Bluelight DNA Dye 10000X

VWR® EZ-Vision® Bluelight DNA Dye 10000X

Supplier: VWR Chemicals

EZ-Vision BlueLight is a sensitive, non mutagenic and environmentally safe fluorescent dye designed for gel staining.

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VWR®, Loading dye base

VWR®, Loading dye base

Supplier: VWR Chemicals

This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.

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VWR®, Nucleic acid loading dyes

VWR®, Nucleic acid loading dyes

Supplier: VWR Chemicals

Designed to optimise loading nucleic acids in agarose gels, the loading dye imparts colour to the sample to facilitate the loading process and increase the density of the sample to ensure efficient sample distribution into each well. The dye migrates independently from the sample, allowing estimation of the migration of nucleic acids.

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J.T.Baker®, Cervix Fixative

Supplier: Avantor

Microscopy and staining reagents, J.T.Baker®, Cervix spray fixative

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Electrophoresis gel stain, Reversible Zinc Stain™

Electrophoresis gel stain, Reversible Zinc Stain™

Supplier: G-Biosciences

A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.

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J.T.Baker®, Mounting Medium, High, Histo Grade

Supplier: Avantor

Mounting medium for coverslipping of slides.

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Differentiation Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

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Osteo-Bed Bone Embedding Solvent

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

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VWR® Q Path® Safemount, Mounting Agents for Microscopy

VWR® Q Path® Safemount, Mounting Agents for Microscopy

Supplier: VWR Chemicals

Safemount Q Path® is a mounting medium, supplied in an PET bottle, which is compatible with Safesolv. It glues the coverglass to the slide and fixes the probe in position after staining.

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VWR® Q Path® O.C.T. Compound Mounting Medium for Cryotomy

VWR® Q Path® O.C.T. Compound Mounting Medium for Cryotomy

Supplier: VWR Chemicals

High viscosity mounting medium for cryotomy.

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Electrophoresis gel stain, Reversible Copper Stain™

Electrophoresis gel stain, Reversible Copper Stain™

Supplier: G-Biosciences

The Reversible Copper Stain™ is a single step stain for the rapid detection of proteins resolved on SDS-PAGE.

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J.T.Baker®, Formalin Neutraliser

Supplier: Avantor

Formalin (dissolved formaldehyde) is used in histopathology laboratories.

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VWR® Fixing Solution

VWR® Fixing Solution

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

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VWR®, D.F.B (Decalcifying solution - H.K. Kristenson) Histological grade

VWR®, D.F.B (Decalcifying solution - H.K. Kristenson) Histological grade

Supplier: VWR Chemicals

Decalcifier according to Kristenson.

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Aqueous mounting medium Aquatex®, Sigma-Aldrich®

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

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VWR®, Immersion Oil

VWR®, Immersion Oil

Supplier: VWR Collection

Immersion oil is used to increase microscope resolution.

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VWR® HistoChoice®, Clearing agent

VWR® HistoChoice®, Clearing agent

Supplier: VWR Chemicals

HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.

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VWR® Gurr™ Histological Fixatives, AFA

VWR® Gurr™ Histological Fixatives, AFA

Supplier: VWR Chemicals

Acidified alcoholic formalin for the fixation of glycogen.

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J.T.Baker® Ultrakitt, Mounting Medium, Histo Grade

Supplier: Avantor

Mounting medium for coverslipping of slides.

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VWR® Bouin's solution

VWR® Bouin's solution

Supplier: VWR Chemicals

VWR® Bouin tissue fixative solution.

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VWR®, OCT Embedding matrix for frozen sections

VWR®, OCT Embedding matrix for frozen sections

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

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VWR® Q Path® DC3, Decalcifier Rapid-Acting

VWR® Q Path® DC3, Decalcifier Rapid-Acting

Supplier: VWR Chemicals

Contains a mixture of hydrochloric acid and alcohols with some EDTA.

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VWR® Q Path® Easyfix, Cytology Fixative

VWR® Q Path® Easyfix, Cytology Fixative

Supplier: VWR Chemicals

Cytology fixative specially designed for the conservation and transport of cytology specimens.

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